Shulou(Shulou.com)06/01 Report--

Today, I will talk to you about the circular RNA annotations and primer design tools in circPrimer, which may not be well understood by many people. in order to make you understand better, the editor has summarized the following contents for you. I hope you can get something according to this article.

There are many software to predict ring RNA based on RNA_seq data, but there are few tools to provide ring RNA annotations. CircPrimer is a software that can annotate circular RNA. By inputting the genome location of circular RNA, you can determine its source gene, extract genome and transcript sequences, and design primers to verify circular RNA.

The software provides a graphical interface and is easy to operate. The classic application scenarios are as follows.

1. Annular RNA comment

As shown below, enter the genome region of the circular RNA and click the Annotate button to view the source gene and sequence composition of the circular RNA

By selecting the corresponding genome version, the DNA/RNA sequence corresponding to the circular RNA can be extracted by circRNA SEQ. It is also supported to enter the circular RNA ID in circBase or the source gene gene symbol. Clicking the circBase button at this time will retrieve it from the database and give the corresponding circular RNA result in the right panel.

two。 Visualization of annular RNA

Click on the retrieved ring RNA and you can see the visualization results shown below

3. Design PCR primers

After you get the RNA sequence, check the Divergent primer and check primer check boxes to view the corresponding primer sequence. The results are as follows

The graphical interface tool is easier to operate, but the disadvantage is that it is only applicable to the annular RNA annotations of the human species.

After reading the above, do you have any further understanding of the circular RNA annotation and primer design tools in circPrimer? If you want to know more knowledge or related content, please follow the industry information channel, thank you for your support.

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