Shulou(Shulou.com)06/01 Report--

This article mainly explains "how to use clusterProfiler in R language to enrich grapes with GO". Interested friends may wish to have a look at it. The method introduced in this paper is simple, fast and practical. Let's let the editor take you to learn how to use clusterProfiler in R language to enrich grapes with GO.

The basic process is to compare Hiast2 to bamstringtie assembly transcript gffcompare to compare the gtf file output from stringtie with the annotation file of the reference genome to get a merged.combine.gtf that uses merged.combine.gtf to calculate the expression for each sample, and the output file is stored in the ballgown folder. The command used in this step is stringtie-e-B-p 8-G merged.combined.gtf-o ballgown/L01/L01.gtf output_bam/L01.sorted.bamimage.png, followed by the R language ballgown packet reading data to obtain the gene and transcript expression code is library (ballgown).

Library (genefilter)

Library (dplyr)

Pheno_data

Welcome to subscribe "Shulou Technology Information " to get latest news, interesting things and hot topics in the IT industry, and controls the hottest and latest Internet news, technology news and IT industry trends.