Shulou(Shulou.com)12/24 Report--

CTOnews.com, December 9 (Xinhua) following the reduction of carbon dioxide to glucose and fatty acids in 2022, the research team of Shenzhen Institute of Advanced Technology of the Chinese Academy of Sciences has made another important breakthrough in the field of synthetic sugar derivatives.

The results were published in the journal Nature Catalysis on December 5.

The research team developed a yeast cell platform by means of synthetic biology and metabolic engineering to convert low-carbon compounds derived from carbon dioxide such as methanol, ethanol and isopropanol into sugars and sugar derivatives such as glucose, inositol, glucosamine, sucrose and starch.

The research team uses low-carbon compound C1-3 as fermentation raw material, which provides a solution with unlimited potential for the sustainable production of food and chemicals by microorganisms.

In this study, the team first constructed a yeast factory with a wider range of carbon sources by analyzing the yeast's utilization of different low-carbon compounds.

The team then regulated the mixed use and proportion of carbon sources to further improve yeast cell growth and glucose production.

Using engineering Pichia pastoris, the scientific team can efficiently convert methanol (C1) into glucose, with a yield of 1.08 grams per liter in shaking flasks and 13.41 grams per liter in fermentors.

Using ethanol, methanol, isopropanol and glycerol as carbon sources, the team further expanded the diversity of carbohydrates, including five-carbon xylose, xylitol, six-carbon inositol, glucosamine, disaccharide sucrose and polysaccharide starch.

Through the introduction of metabolic engineering and heterosynthesis pathway, engineering yeast can successfully convert low-carbon compounds into monosaccharide xylose, xylitol, inositol and glucosamine. The highest yield of inositol and glucosamine in shake flask reached 228.71mg / L and 69.99 mg / L, respectively.

In addition to monosaccharides, the researchers also achieved the synthesis of disaccharides with higher carbon content. In this study, by introducing sucrose biosynthesis pathway and strengthening endogenous metabolic flux, the engineering strain could efficiently use low carbon compounds as carbon source to synthesize sucrose. On this basis, sucrose transporter was expressed. The secretory production of sucrose was realized. The yield of sucrose can reach 1.17 g / L in shaking flask and 25.41 g / L in fermentation.

What is even more exciting is that the researchers have achieved the microbial synthesis of starch involved in all aspects of our lives, and its importance is self-evident. In this study, two starch synthesis pathways and regulation of endogenous glycogen synthesis and degradation pathways were introduced to open up the synthesis pathway of starch from low-carbon compounds, and the yield of starch in shaking flask could reach 341.59mg / L. These research results realize the "agricultural production" of microorganisms.

The reference address of the paper is attached to CTOnews.com:

Tang, H., Wu, L., Guo, S. Et al. Metabolic engineering of yeast for the production of carbohydrate-derived foods and chemicals from C1-3 molecules. Nat Catal (2023). Https://doi.org/10.1038/s41929-023-01063-7

Nature Catalysis | preparation of food compounds from low-carbon raw materials derived from carbon dioxide

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