Shulou(Shulou.com)06/02 Report--

This article will explain in detail how to realize the difference analysis operation between the two groups in DESeq2. The content of the article is of high quality, so the editor will share it with you for reference. I hope you will have a certain understanding of the relevant knowledge after reading this article.

1. Read data

Read the expression table of the gene and the grouping information of the sample, among which the example of the expression file is as follows

Gene_id ctrl-1 ctrl-2 ctrl-3 case-1 case-2 case-3geneA 14 0 11 40 12geneB 125 401 442 175 59 200

Each behavior has a gene, and each column represents a sample.

Examples of files for grouping information are as follows

Sample conditionctrl-1 controlctrl-2 controlctrl-3 controlcase-1 casecase-2 casecase-3 case

The first column is the sample name, and the second is the grouping information of the sample.

The code to read the file is as follows

# read the expression table count

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