Shulou(Shulou.com)11/24 Report--

CTOnews.com, September 11 (Xinhua) the University of Bielefeld in Germany has developed a fluorescence microscope that uses structural lighting for fast super-resolution imaging in a wide field of view, which focuses on "observing living cells" and supports "high-speed imaging". It is said that "high-resolution images of multiple living cells can be captured at the same time, helping to analyze the effects of different drugs and their combinations on the human body."

Researcher Henning Ortkrass claims that the microscope is part of an EIC Pathfinder OpenProject DeLIVERY project to study drugs for patients with chronic diseases and the elderly, which aims to develop a platform to study multiple drug use in individual patients.

The microscope currently deployed by ▲ University of Bielefeld in Germany uses fluorescent lighting, so it is more suitable for "observing living cells." it is reported that this new type of microscope is based on super-resolution structure illumination microscope (SR-SIM), which uses structured light mode to excite fluorescence in samples to achieve spatial resolution beyond the limit of light diffraction. the microscope is quite suitable for living cell imaging and uses low power consumption excitation. On the other hand, the power consumption of traditional microscope imaging is high, which is easy to harm the sample itself.

In order to achieve high resolution with wide field of view, a new type of microscope uses AI to reconstruct super-resolution images from a group of original images. These original images are obtained by irradiating the sample with a sinusoidal stripe pattern using a set of six optical fibers, resulting in a tripling of resolution, fast imaging and compatibility with living cell imaging.

CTOnews.com after query, the relevant results have been published in the Optica Publishing Group's "Optical KuaiBao" (Optics Express) magazine, studies have shown that the instrument in the liver imaging testing, the field of view of 150 x 150 μ m ², the imaging rate of 44 Hz, while maintaining a space-time resolution of less than 100 nm.

▲ Eye is the imaging of hepatocytes shown in the journal, and the source Optics Express researchers also demonstrated in the journal the imaging of fixed multicolor stained hepatocytes with a new microscope device. The image shows the tiny membrane structure of the cell, which is less than the diffraction limit of light.

Optical KuaiBao claims that with this new microscope, researchers can test individual drug combinations on cells in vitro and then perform super-resolution imaging to observe the dynamic changes of cell membrane characteristics or organelles. A large field of view can provide statistical information about cell response, which can be used to improve personalized health care, and it can also be used in very important high-resolution clinical applications.

The researchers next plan to apply the microscope to the study of living cells of hepatocytes to observe the dynamic changes of cells treated with multiple drugs. They also plan to improve the image reconstruction process to complete the real-time reconstruction of the original data.

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