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This article mainly introduces the blastall file format in what the relevant knowledge, the content is detailed and easy to understand, the operation is simple and fast, with a certain reference value, I believe you read this blastall file format of what articles will have a harvest, let's take a look at it.

1. Query id: query sequence ID identification

2. Subject id: target sequence ID identification on alignment

3.% identity: percentage of consistency of sequence alignment

4. Alignment length: the length of the alignment area that matches the alignment

5. Mismatches: the number of mismatches in the comparison area

6. Gap openings: compare the number of gap in the region

7, Q. start: the starting point of the alignment region on the query sequence (Query id)

8. Q. end: the termination site of the alignment region on the query sequence (Query id)

9. S. Start: the starting site of the alignment region on the target sequence (Subject id)

10. S. End: the termination site of the alignment region on the target sequence (Subject id)

11. E-value: the expected value of the comparison result. The explanation is about how many random comparisons can occur. The smaller the score,Evalue, the less likely it is to happen in terms of probability. If it happens, it means it is more likely to be a real similar sequence.

12. Bit score: compare the bit score value of the result

In general, we look at columns 3, 11 and 12. The smaller the e value is, the more reliable it is.

The parameter corresponding to blast is-m 8

The parameter corresponding to blast+ is-outfmt 6

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