Shulou(Shulou.com)06/02 Report--

This article will explain in detail what is the use of metascape database, the content of the article is of high quality, so the editor will share it with you for reference. I hope you will have a certain understanding of the relevant knowledge after reading this article.

Enrichment Analysis of metascape Database

Introduction: in bioinformatics research, the need for GO and KEGG enrichment analysis to obtain gene lists is very common. At present, there are many bioinformatics methods or databases that can realize gene enrichment analysis, such as DAVID, but some of them are charged, some are not easy to use and rarely maintain. For example, DAVID did not maintain the database for six years (2010-2016), and the most recent update has been two and a half years. Metascape updates its more than 40 related databases every month to ensure that the most accurate results are provided. Therefore, Metascape database can be used as a better means of enrichment analysis.

Step1: paste a gene list or upload a gene file

Step2: setting species

Step3: point Express Analysis

The default Express Analysis mixes the signal pathways of many databases, resulting in all kinds of redundancy. For example, by default, Reactome, KEGG, Hallmark and GO databases are all displayed together, but usually when we draw scientific research, we will show a picture of GO and a picture of KEGG respectively.

So in the enrichment option in custome Analysis, from bottom to top, select the database related to GO, then check pick selective, and then click enrichment analysis.

What is the use of the metascape database to share here, I hope the above content can be of some help to you, can learn more knowledge. If you think the article is good, you can share it for more people to see.

Welcome to subscribe "Shulou Technology Information " to get latest news, interesting things and hot topics in the IT industry, and controls the hottest and latest Internet news, technology news and IT industry trends.