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How to use gene enrichment analysis online tool metascape, I believe that many inexperienced people do not know what to do, so this paper summarizes the causes of the problem and solutions, through this article I hope you can solve this problem.

Metascape is a web tool, which provides many functions, such as gene enrichment analysis, protein interaction network analysis and so on. The corresponding article is published in nature. On communications

The website integrates more than 40 gene function annotation databases and provides a variety of visualization ways. for biologists, gene function can be easily explored and analyzed through the site. So far, 451 articles have cited the tool, and its visualization is as follows

1. Circos plot

2. Enrichment Bar Graph

3. Enrichment Heatmap

4. Enrichment Network

5. Protein Complex Network

6. Membership Pie Chart

The integrated functional annotation database is as follows

As shown in the figure above, the database annotated with these functions mainly carries out the following analyses

ID Conversion

Gene Annotation

Enrichment Analysis

Interaction Network Analysis

For a single gene list, enrichment analysis and PP analysis are performed by default, as shown below, enter the gene list, select the species, and then click Express Analysis.

The results include the following two types

1. Histogram of enrichment analysis

By default, GO, KEGG, Reactome, CORUM and other databases are used for enrichment analysis. The results are as follows.

Show the significantly enriched terms of top15 in the form of a bar chart, as shown below

The relationship between the enriched terms is shown in the form of a network diagram, as shown below

2. PPI analysis

The protein-protein interaction network diagram is provided, and the PPI network is clustered by MCODE algorithm to identify the subnetwork, that is, the potential protein complex. The results are as follows.

If multiple genes are entered at the same time, two additional visualization methods are provided. The first is the display of multiple enrichment results in the form of a heat map, as shown below.

The second is the overlap analysis between the input multiple gene sets. The results are shown by circos diagrams. The lnks connections between the two regions represent the corresponding overlap. The results are shown below.

Metascape integrates the most databases and is updated in a timely manner. Through custom analysis, the functional analysis of multiple databases can be realized. The database currently supports gene analysis of 10 species, including humans, mice, rats, etc., and is a very powerful tool for gene function analysis.

After reading the above, have you mastered how to use metascape, an online tool for gene enrichment analysis? If you want to learn more skills or want to know more about it, you are welcome to follow the industry information channel, thank you for reading!

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