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Most people do not understand the knowledge points of this article "iTOL how to add circles to the outer layer of the evolutionary tree", so the editor summarizes the following content, detailed content, clear steps, and has a certain reference value. I hope you can get something after reading this article. Let's take a look at this article "how iTOL adds circles to the outer layer of the evolutionary tree".

First of all, prepare the evolutionary tree.

Then prepare the comment file in the following format:

DATASET_COLORSTRIP#lines starting with a hash are comments and ignored during parsing#select the separator which is used to delimit the data below (TAB,SPACE or COMMA). This separator must be used throught this file (except in the SEPARATOR line, which uses space). # SEPARATOR TABSEPARATOR SPACE#SEPARATOR COMMA#label is used in the legend table (can be changed later) DATASET_LABEL color_strip2#dataset color (can be changed later) COLOR # ff0000#optional settings#all other optional settings can be set or changed later in the web interface (under 'Datasets' tab) # maximum widthSTRIP_WIDTH 25#left margin Used to increase/decrease the spacing to the next dataset. Can be negative, causing datasets to overlap.MARGIN 0#border width; if set above 0, a black border of specified width (in pixels) will be drawn around the color strip BORDER_WIDTH 1BORDER_COLOR # 000#show internal values; if set, values associated to internal nodes will be displayed even if these nodes are not collapsed. It could cause overlapping in the dataset display.SHOW_INTERNAL 0#In colored strip charts, each ID is associated to a color. Color can be specified in hexadecimal, RGB or RGBA notation#Internal tree nodes can be specified using IDs directly, or using the 'last common ancestor' method described in iTOL help pages#Actual data follows after the "DATA" keywordDATA#ID1 value1#ID2 value2160232 # efff22 COL#efff2213773 # efff22 COL#efff2256636 # efff22 COL#efff22111955 # efff22 COL#efff222287 # efff22 COL#efff2250339 # efff22 COL#efff222303 # efff22 COL#efff222234 # efff22 COL#efff22

The previous parts of DATA are all format statements, which can be copied. Then add the torus data, which is divided into two columns, the first column is the original label, and the second column is the corresponding color of the torus, separated by Ta spaces. When the data is ready, it can be added to the evolutionary tree.

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