Shulou(Shulou.com)06/01 Report--

This article is about how BLAST+ works. Xiaobian thinks it is quite practical, so share it with everyone for reference. Let's follow Xiaobian and have a look.

BLAST+ is a very widely used sequence alignment software, provided by NCBI.

BLAST+ is a very widely used sequence alignment software, available from NCBI and available for download at:

ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/

The method of use is as follows:

formatted database

makeblastdb -in db.fasta -dbtype prot -parse_seqids -out dbname

Parameter description:

-in: sequence file to be formatted

-dbtype: database type, prot or nucl

-out: database name

Protein sequence alignment protein database (blastp)

blastp -query seq.fasta -out seq.blast -db dbname -outfmt 6 -evalue 1e-5 -num_descriptions 10 -num_threads 8

Parameter description:

-query: Enter file path and file name

-out: Output file path and file name

-db: formatted database path and database name

-outfmt: output file format, there are 12 formats in total, 6 is tabular format corresponding to BLAST m8 format

-evalue: Set the e-value of the output result

-num_descriptions: the number of tabular output results

-num_threads: Number of threads

Nucleic acid sequence alignment nucleic acid database (blastn) and nucleic acid sequence alignment protein database (blastx)

Similar to blastp above:

blastn -query seq.fasta -out seq.blast -db dbname -outfmt 6 -evalue 1e-5 -num_descriptions 10 -num_threads 8

blastx -query seq.fasta -out seq.blast -db dbname -outfmt 6 -evalue 1e-5 -num_descriptions 10 -num_threads 8

Thank you for reading! About "BLAST+ how to use" this article is shared here, I hope the above content can be of some help to everyone, so that everyone can learn more knowledge, if you think the article is good, you can share it to let more people see it!

Welcome to subscribe "Shulou Technology Information " to get latest news, interesting things and hot topics in the IT industry, and controls the hottest and latest Internet news, technology news and IT industry trends.