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Today, I will talk to you about the example analysis of the HACER human enhanced subdatabase, which may not be well understood by many people. in order to make you understand better, the editor has summarized the following content for you. I hope you can get something according to this article.

Most enhancer databases use chip_seq,DNase_seq and CAGE_seq data to identify enhancers, while HACER uses GRO_seq and PRO_seq to analyze enhancer RNA, so as to identify activated enhancers.

Compared with other enhancements, the situation is as follows

The web address of the database is as follows

Http://bioinfo.vanderbilt.edu/AE/HACER/

For the identified enhanced subregions, the database also provides the following three regulatory relationships

TF-Enhancer

Enhancer-target gene

Enhancer-promoter

At the same time, for the genome region corresponding to the enhancer, the information of GWAS SNP and eQTL mutation sites in this region is also provided, as shown below.

Because of the tissue and cell specificity of enhancers, the enhancer information in different cell lines can be viewed through the Browser menu in this database, as shown below

Check the check box in front of the interested cell line. Take GM12878 as an example, the search results are as follows

Click the enhancer ID to view the details. Take AE_hg19_GM12878_ 20878` as an example, the results are as follows

1. Basic

2. TF binding

3. Target Gene

4. GWAS

5. EQTL

The information in this database is downloaded free of charge. Through this database, we can easily explore the regulatory network of enhancers, TF and genes, and combine the mutation information to combine the multi-level and multi-group data of genome and transcriptional regulation to provide more reference information for exploring the specific mechanism.

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