Shulou(Shulou.com)06/02 Report--

How to use monocle package for pseudotime analysis, in view of this problem, this article introduces the corresponding analysis and solution in detail, hoping to help more partners who want to solve this problem to find a more simple and feasible method.

Monocle is an R packet specially used to analyze single-cell transcriptome data, providing clustering, pseudotime, difference analysis and other functions.

This paper mainly introduces the steps of using the R packet for pseudotime analysis, which can be divided into the following five steps

1. Read data

There are many ways to read data in the monocle package. Here we only show how to read objects in Seurat. The code is as follows

# load the required R package library (Seurat) library (monocle) # set the cell ranger output result directory input_dir

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