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Editor to share with you what is the use of VISTA database, I believe most people do not know much about it, so share this article for your reference, I hope you can learn a lot after reading this article, let's go to know it!

VISTA combines comparative genomics, chip_seq and other data analysis methods and experimental methods to identify enhancers in humans and mice. The website of the database is as follows

Http://enhancer.lbl.gov/

In the initial study, it was found that half of the conserved regions among species such as humans and mice contain enhancers, so identifying conserved genome sequences among species by means of comparative genomics has a certain reference significance for enhancer analysis; at the same time, many enhancer regions can also be identified by using the chip_seq results of EP300 protein.

Through the combination of the above two bioinformatics analysis methods, the candidate enhancer sequences were first identified, and then the enhancer activity was verified by transgenic mice experiments.

Using the retrieval function of the home page, you can view the records in the database, as shown below

It can be searched by chromosome interval, or by other methods such as gene name. The search results are as follows

The number of the enhancer in the database, the position on the genome, the gene contained and other information are given. The mouse icon on the right represents the information of the experiment outside the enhancer, and the blue represents that the enhanced activity of the enhancer is confirmed by experimental means. White means that the enhanced activity of the enhancer has not been found in the in vitro experiment. Click the number in the first column to view the details.

At the same time, considering the tissue specificity of enhancers, the expression of enhancers in different tissues was studied by tissue section technique, and the relevant information of the following tissues was provided.

Forebrain

MidBrain

Hindbrain

Neural Tube

Eye

Ear

Dorsal Root Ganglia

Trigeminal

Cranial Nerves

Heart

Tail

Limb

Nose

Face

Branchial Arch

Through Advance Search, enhancers can be screened according to their expression information in different tissues, as shown below

The enhancer information of human and mouse can be easily searched through VISTA database.

The above is all the contents of the article "what is the use of VISTA database". Thank you for reading! I believe we all have a certain understanding, hope to share the content to help you, if you want to learn more knowledge, welcome to follow the industry information channel!

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