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In this article, the editor introduces in detail "how to use qiime2 to split data on barcode information", the content is detailed, the steps are clear, and the details are handled properly. I hope that this article "how to use qiime2 to split data on barcode information" can help you solve your doubts.

First, prepare the sample-metadata.tsv file and the barcode file information of the sample. Here, take double-end sequencing as an example:

Sample-id forward-barcodes reverse-barcodesLin027 GATCTGCA CTACGATGLin028 GATCTGCA GACATAGCLin029 GATCTGCA GATCTGCALin032 GATCTGCA GCGTATGALin033 GATCTGCA GTATGCGA

To prepare the sequencing data, put the unsplit double-ended data into a directory: muxed-pe-barcode-in-seq, which is named forward.fastq.gz reverse.fastq.gz.

Data Import:

Qiime tools import-type MultiplexedPairedEndBarcodeInSequence\-input-path muxed-pe-barcode-in-seq\-output-path multiplexed-seqs.qza

Use the qiime cutadapt plug-in to split the data:

Qiime cutadapt demux-paired-- i-seqs multiplexed-seqs.qza\-- m-forward-barcodes-file sample-metadata.tsv\-- m-forward-barcodes-column forward-barcodes\-m-reverse-barcodes-file sample-metadata.tsv\-- m-reverse-barcodes-column reverse-barcodes\-- o-per-sample-sequences per_sample_sequences.qza\-- o-untrimmed-sequences untrimmed_sequences.qza

The per_sample_sequences.qza file is split data, and the barcode sequence has been removed.

If you want to remove primers, you can use:

Qiime cutadapt trim-paired\-- i-demultiplexed-sequences per_sample_sequences.qza\-- p-cores 4\-- p-no-indels\-- p-front-f ACTCCTACGGGAGGCAGCAG\-- p-front-r GGACTACHVGGGTWTCTAAT\-- o-trimmed-sequences primer-trimmed-demux.qza reads here, this article "how to use qiime2 to split barcode information into data" has been introduced. If you want to master the knowledge of this article, you still need to practice and use it. If you want to know more about the articles, you are welcome to follow the industry information channel.

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