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This article mainly introduces the use of MirSNP database, the article is very detailed, has a certain reference value, interested friends must read it!

In the field of SNP research, a large number of SNP loci related to diseases or some complex traits have been found by means of GWAS or eQTL, and the specific functions of these SNP need to be further analyzed and mined. Some scientists have found that SNP loci can cause the occurrence and development of diseases by affecting miRNA, so it is very meaningful to study miRNA-related SNP loci.

The MirSNP database is a database that stores miRNA-related SNP sites at the following URL

Http://bioinfo.bjmu.edu.cn/mirsnp/search/

The database contains two kinds of miRNA-related SNP sites, one is the SNP site located on the gene of pre-miRNA, these SNP sites directly affect the generation of miRNA, the analysis process is as follows

The SNP loci located in this region were screened out directly according to the chromosome location of miRNA precursor gene.

The other is the SNP sites in the miRNA collection area discussed at the beginning. These SNP sites may affect the binding of miRNA to mRNA. The analysis process is as follows.

According to the miRNA binding region information predicted by miranda software, the SNP sites located in the binding region were found.

The official website provides a variety of search functions. Take single search as an example to illustrate. The search box is as follows.

Multiple query data such as Gene, mRNA, SNP and miRNA are supported. SNP loci can be screened according to the frequency of MAF in different populations. Bingding map will display the details of the binding areas of miRNA and mRNA. The search results are as follows

MirSVR is the score of miRNA binding given by mirRanda software, Effect is to analyze the influence of SNP site on miRNA collection, create represents the occurrence of SNP leading to the existence of miRNA binding site on mRNA, enhance represents the occurrence of SNP leads to more stable miRNA binding region, break represents the occurrence of SNP leads to the loss of miRNA binding site, and decrease represents the occurrence of SNP leading to the decline of the stability of miRNA collection region.

The score value is the score of the stability of the miRNA binding region given by miRanda. The larger the value is, the more stable the complex structure formed by the combination of miRNA and mRNA is. Energy represents the free energy of the complex, and conservation represents the conservative score of the binding region.

The miRBase that this database depends on, and the version of the dbSNP database is shown below

Now, the version is relatively old, but its analysis ideas are still worthy of reference. We can use the information in the new version of the database to sort out the miRNA-related SNP locus information.

The above is all the contents of the article "what is the use of MirSNP database". Thank you for reading! Hope to share the content to help you, more related knowledge, welcome to follow the industry information channel!

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